乙型肝炎病毒对精子染色体的影响

doi:10.3969/j.issn.1004-616x.1999.02.003

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (2): 72-074.doi: 10.3969/j.issn.1004-616x.1999.02.003

Previous Articles Next Articles

THE EFFECT OF HEPATITIS B VIRUS ON HUMAN SPERM CHROMOSOME

Huang J ianmin1 , Huang Tianhua1 , Qiu Huanying2 , Qiu J iewen3 , Fang Xiaowu1 , Chen Xiaohong1 , Zhung Tiangang1 , Cai Shushen1 , Wang Yonghua2 , Liu Hongxi1 , Yang Fengxia2 , Liu Weidong2

1L aboratory of Reproductive Biology , S hatou University Medical College , S hantou 515063 , 　2 S hangtou University Hospital , 　3 The Inf ectious Department of the Second A f f iliated Hospital , S hantou University Medical College

Received:1998-09-15 Revised:1998-11-20 Online:1999-03-30 Published:1999-03-30

Abstract

Abstract: Sperm chromosome of subject s with hepatitis B virus infection were prepared using interspecific in vit ro fertilization with zona2f ree hamster eggs. Fluorescence in situ hybridization ( FISH) to sperm chromosome spreads was carried out with biotin2labelled full length HBV DNA. There was significant difference in the frequencies of sperms with chromosome aberrations between subject s with HBV infection and cont rol. FISH showed that HBV DNA integrated into sperm chromosomes , and that multisite and nonspecific integrations were the features of HBV in sperm chromosomes. These result s indicate that HBV infection affect s on the sperm chromosome broadly.

Key words: hepatitis B virus, human sperm chromosome, zona2f ree hamster egg, FISH, chromosome aberration, DNA integration, vertical t ransmission

Huang J ianmin , Huang Tianhua , Qiu Huanying , Qiu J iewen , Fang Xiaowu , Chen Xiaohong , Zhung Tiangang , Cai Shushen , Wang Yonghua , Liu Hongxi , Yang Fengxia , Liu Weidong. THE EFFECT OF HEPATITIS B VIRUS ON HUMAN SPERM CHROMOSOME[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(2): 72-074.

[1]	DU Xiuming, HONG Liling, XU Lingzhi, ZHOU Qingyun. In vitro chromosome aberration evaluation of anatase titanium dioxide nanoparticles [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 67-71.
[2]	YU Zhiqiang, SUN Yun, CHEN Xiaoqing, LIU Hanwei, MA Zhongchun. Establishment of an in vitro biotoxicity detection method of paralytic shellfish poisoning [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 323-326.
[3]	WU Desheng, WANG Honghui, YUAN Jianhui, WANG Hongju, ZHAO Qionghui, LIU Jianjun. Effect of fine particle exposure on development of zebrafish embryos [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 307-309,314.
[4]	SHEN Biling, GUO Xiaoling, HE Yun. Establishment of a CCM3 gene knockdown zebrafish model and its preliminary application in toxicology [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(3): 194-198,204.
[5]	YUAN Ye, CHEN Dandan, JING Shufang, YU Yongsheng, CHEN Jianheng, WU Chunqi, SHI Chang. Mutagenic effects of N-(n-butyl)-thiophosphorictriamide in somatic and germ cells of mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 432-437.
[6]	GAO Mei, CAO Chong, YING Yong, MA Hui, JIA Qingwen. Comparison of different inductionmethods on activity of rat liver microsomal enzymes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 269-272.
[7]	YING Jian, HAO Binxiu, MENG Qingjia, CHEN Ran, WANG Liming, WANG Chunling. Acute toxicity and genotoxicity of crude water extracts from tea mistletoe Viscum Liquidambaricolum Hayata [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 306-309,324.
[8]	LUO Xueting, CAI Qijun, ZHAO Bo, CHEN Weimin, CHEN Xiao, ZHANG Xiaowen. Evaluation of acute and genetic toxicity of Zihe herbal solid beverage in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 302-305.
[9]	SUN Ye, LIU Wei, ZHENG Jian, LÜ Yuan, HUANG Haiyan, PAN Liubo, WANG Zhou, HUANG Wei, LIU Jianjun. Biotoxin of shellfish products in Shenzhen city from 2011 to 2014 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(2): 149-150,154.
[10]	LI Xuling, WU Desheng, HONG Wenxu, LIU Jianjun, KE Yuebin, YUAN Jianhui, LI Shuangfei. Effects of nano-SiO₂ particles on zebrafish behavior response [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 467-471,476.
[11]	KANG Xiangjin, DING Yue, YANG Jie, DU Hongzi, LIU Jianqiao, HUANG Tianhua. Effects of hepatitis B virus S protein exposure on human sperm oxidative stress [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 202-206.
[12]	YIN Jian，WANG Ai-ping，LI Wan-fang，JIN Hong-tao，WEI Jin-feng. Establishment and application for an oxidative stress biomarker quantitative analysis system [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(5): 321-329.
[13]	LI Shuang，LU Xue，ZHAO Hua，FENG Jiang-bin，LIU Qing-jie. Establishment of a rapid FISH method to analyze chromosome aberrations induced by Irradiation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(2): 123-126.
[14]	LIU Qiang，LI Ke-qiu，DU Li-qing，WANG Yan，XU Chang，FAN Sai–jun，LI Guang，Markus Hecker. Genetoxicity to human beings caused by the pollution of electronic wastes recycling [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(6): 448-453.
[15]	CHEN Ying，LI Ying，MA Ai-cui，XU Li，PAN Qi，LIU Xiang-yun，ZHOU Li，SUN Zu-yue. Comparetive study of different solvent toxicity on CHL cells in chromosome aberration test [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(5): 373-376.

THE EFFECT OF HEPATITIS B VIRUS ON HUMAN SPERM CHROMOSOME

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments